Development of real-time NASBA assays with molecular beacon detection to quantify mRNA coding for HHV-8 lytic and latent genes
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چکیده
منابع مشابه
Development of real-time NASBA assays with molecular beacon detection to quantify mRNA coding for HHV-8 lytic and latent genes
BACKGROUND Human herpesvirus-8 (HHV-8) is linked to the pathogenesis of Kaposi's sarcoma (KS), and the HHV-8 DNA load in peripheral blood mononuclear cells (PBMC) is associated with the clinical stage of KS. To examine the expression of HHV-8 in PBMC, four HHV-8 mRNA specific NASBA assays were developed METHODS We have developed four quantitative nucleic acid sequence-based amplification assa...
متن کاملmolecular beacon probes-base multiplex nasba real-time for detection of hiv-1 and hcv.
developed in 1991, nucleic acid sequence-based amplification (nasba) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than pcr, and it can also be more sensitive. this paper describes the development of a molecular beacon-based multiplex nasba assay for simultaneous detection of hiv-1 and hcv in plasma samples.a well-conserved region...
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BACKGROUND AND OBJECTIVES Developed in 1991, nucleic acid sequence-based amplification (NASBA) has been introduced as a rapid molecular diagnostic technique, where it has been shown to give quicker results than PCR, and it can also be more sensitive. This paper describes the development of a molecular beacon-based multiplex NASBA assay for simultaneous detection of HIV-1 and HCV in plasma sampl...
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A molecular beacon-based real-time NASBA (QNASBA) assay for detection and identification of Listeria monocytogenes has been developed. A correlation between targeting highly accessible mRNA sequences and QNASBA efficiency and sensitivity was demonstrated. The assay targets a sequence from the mRNA transcript of the hly gene which is specific for this bacterium; and includes an internal amplific...
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ژورنال
عنوان ژورنال: BMC Infectious Diseases
سال: 2002
ISSN: 1471-2334
DOI: 10.1186/1471-2334-2-18